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Synthesis of 2‐Deoxy‐maltooligosaccharides with Phosphorylase and Their Degradation with Amylases
Author(s) -
Evers Britta,
Thiem Joachim
Publication year - 1995
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19950471107
Subject(s) - chemistry , substrate (aquarium) , glycogen phosphorylase , enzyme , phosphate , amylase , mass spectrometry , degradation (telecommunications) , substrate specificity , biochemistry , chromatography , biology , ecology , telecommunications , computer science
By reaction of phosphorylase with D‐glucal and inorganic phosphate instead of the natural substrate α‐D‐glucosyl phosphate novel 2‐deoxy‐maltooligosaccharides could be synthesized. Their properties and characterization by NMR spectroscopy, mass spectrometry and HPLC are described. Under similar conditions, except a higher phosphate concentration, 2‐deoxy‐α‐D‐glucosyl phosphate is obtained. This can also serve for the enzymatic elongation of the primer with 2‐deoxy‐glucosyl residues. The unnatural 2‐deoxy‐maltooligomers could be applied successfully as substrates for the exo‐cleaving enzymes glucoamylase and β‐amylase.