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Immobilization of Aspergillus niger Mycelium on Seeds for Glucoamylase Production
Author(s) -
Fiedurek Jan,
Szczodrak Janusz
Publication year - 1995
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19950470508
Subject(s) - aspergillus niger , mycelium , laboratory flask , enzyme , food science , yield (engineering) , chemistry , enzyme assay , biochemistry , biology , horticulture , organic chemistry , materials science , metallurgy
Mycelium of the glucoamylolytic mutant Aspergillus niger C‐58‐III was immobilized on wheat, rye, barley, pea, buckwheat and mustard seeds in repeated‐batch flasks. After every 24th it was possible to obtain culture broth rich in glucoamylase. The highest yield of enzyme (66.4 U × ml −1 ) was obtained on the mustard carrier. Immobilized cells were successfully reused with high level of enzyme formation being mantained for longer period (192h). Some of the variables influencing the enzymatic activity have been standardized. Enzyme productivity reached in immobilized cells of A. niger was 1.6‐times higher in comparison with free cells.