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Biosensor for Determination of Starch
Author(s) -
Vrbová E.,
Pecková J.,
Marek M.
Publication year - 1993
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19930451003
Subject(s) - biosensor , glutaraldehyde , glucose oxidase , immobilized enzyme , chemistry , starch , chromatography , covalent bond , substrate (aquarium) , hydrolysis , enzyme , organic chemistry , biochemistry , oceanography , geology
Biosensor for determination of starch was prepared by fixing a carrier with immobilized enzyme system on the tip of a Clark ‐type oxygen sensor. Enzymes amyloglucosidase, mutarotase, glucose oxidase and catalase were co‐immobilized either on the partially hydrolyzed nylon net or on the activated collagen membrane. In both cases the covalent bond was made in the presence of glutaraldehyde and cyclohexyl isocyanide. The prepared biosensors were compared from the viewpoint of a specific activity of the immobilized amyloglucosidase, of a linear response area of the biosensor to the substrate concentration, of the pH and temperature of the reaction mixture and of the biosensor stability. Biosensors prepared were used for the determination of starch in the samples of cultivaiting media for the monensin production.