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Expression of the CGTase Gene of Alkalophilic Bacillus No. 38‐2 in Various Hosts
Author(s) -
Georganta Georgia,
Kaneko Takahiro,
Kudo Toshiaki,
Horikoshi Koki
Publication year - 1991
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19910430908
Subject(s) - bacillus subtilis , plasmid , shuttle vector , escherichia coli , expression vector , biology , gene , bacillus (shape) , vector (molecular biology) , microbiology and biotechnology , gene expression , bacteria , biochemistry , genetics , recombinant dna
We compared the expression level of cyclomaltodextrin glucanotransferase (CGTase) gene of alkalophilic Bacillus species No. 38‐2 in various hosts. Plasmid pEAP85, a new excretion vector, containing tac promoter for expression was constructed. CGTase gene was introduced into a new excretion vector pEAP85 and a shuttle vector pHY300PLK and plasmids pCS8, pEAP85‐CGT and pKG‐1 were constructed. Escherichia coli carrying pEAP85‐CGT excretes 70% (350 U/ml culture) of total CGTase (500 U/ml) into the culture broth. This amount was higher than that produced by the original Bacillus species. Bacillus subtilis carrying pGK‐1 produced very low level of CGTase(< 0.1 U/ml).