Premium
Determination of Glucoamylase Activity with Glucose Electrode
Author(s) -
Przybyt Malgorzata
Publication year - 1991
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19910430509
Subject(s) - glucose oxidase , starch , chemistry , clark electrode , amylase , electrode , chromatography , enzyme , biochemistry , electrolyte
The method of glucoamylase activity assay by oxygen electrode with immobilized glucose oxidase is described. The optimal conditions of assay were established and are following: 1% starch, pH 4.5, and 25°C. The method could be applied to kinetic measurements of glucoamylase action on starch. The results of such investigations are similar to obtained earlier by chemical method. The method could be used for assay of glucoamylase activity in the presence of α‐amylase by changing the pH of solution to 7.