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Nuclear Magnetic Resonance Studies of Homopolysaccharides Related to Starch
Author(s) -
McIntyre Deane D.,
Vogel Hans J.
Publication year - 1991
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19910430209
Subject(s) - pullulan , inulin , fructan , polysaccharide , dextran , chemistry , branching (polymer chemistry) , hydrolysis , residue (chemistry) , starch , glucan , carbohydrate , nuclear magnetic resonance spectroscopy , biochemistry , stereochemistry , organic chemistry , fructose
One‐ and two‐dimensional 1 H and 13 C NMR spectra were obtained for a variety of homopolysaccharides, including the glucans dextran and pullulan, and the fructans levan and inulin. The NMR results provided information about the primary structure of these polysaccharides. Our results are in agreement with those determined previously by methylation analysis and other classical methods. The number of α(1 → 44) and α(1 → 6) linkages could be quantitated for pullulan. The presence of a single nonreducing glucose residue in the polyfructose inulin could also be demonstrated. In the case of the branched polysaccharides dextran and levan, it was possible to determine the degree of branching. In addition for lower molecular weight polysaccharides such as inulin and partially hydrolyzed dextran, it was possible to detect signals arising from reducing and non‐reducing end groups. Their approximate molecular weight could be determined by quantitation of these signals.