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Glucoamylase Biosynthesis by Cells of Aspergillus niger C 58‐III Immobilized in Sintered Glass and Pumice Stones
Author(s) -
Fiedurek J.,
Lobarzewski J.
Publication year - 1990
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19900420908
Subject(s) - aspergillus niger , spore , extracellular , pumice , fungus , mycelium , chemistry , liquid culture , liquid medium , chromatography , nuclear chemistry , microbiology and biotechnology , botany , biochemistry , biology , paleontology , volcano
A simple method of A. niger C 58‐III cell immobilization is described. This strain produces extracellular glucoamylase. According to the proposed method A. niger spores were first immobilized by adsorption in sintered glass Rasching rings (RR) or pumice stones (PS). Growing out from spores, A. niger cells produced extracellular glucoamylase. This technique facilitates the culture growth in a filamentous spongy structure of the supports with a continuous accumulation of biomass. After every 24 h it was possible to obtain culture liquid rich in glucoamylase. This procedure can be repeated 30 times using the same sample of immobilized A. niger culture without any loss of glucoamylase activity in the liquid medium. In a 96 h period immobilized A. niger cells produced 300 units × ml −1 whereas a shake culture of this fungus produced only 186 units × ml −1 .