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Kinetic Characterization of Chimeric Cyclomaltodextrin Glucanotransferase from Genes of Two Alkalophilic Bacillus
Author(s) -
Kaneko Takahiro,
Nakamura Nobuyuki,
Horikoshi Koki
Publication year - 1990
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19900420907
Subject(s) - enzyme , chimeric gene , biochemistry , gene , chemistry , starch , yield (engineering) , bacillus (shape) , bacillaceae , biology , bacteria , genetics , gene expression , materials science , bacillus subtilis , metallurgy
The chimeric CGTase, which was constructed genetically with two β‐CD forming type enzyme genes of different species of alkalophilic Bacillus, was characterized by comparing with the actions of parent enzymes. The final composition of CDs in the reaction digest from starch by the chimeric enzyme, especially in the yield of α‐CD, was different from those by parent enzymes, and total yields of CDs by these enzymes were almost the same. The chimeric enzyme catalyzed the formation of β‐CD as a major action from starch, the rapid synthesis of α‐CD from other CDs and maltooligosaccharides and the slow degradation and interconversion of α‐CD to others. From these results, the role of second and third segments in the enzyme molecules was discussed.