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An Enzymatic Assay Method for Bacterial Alpha‐amylase Using a Glucose Determination‐kit and Maltohexaose as the Substrate
Author(s) -
Ohnishi Masatake,
Iwata Ken,
Hiromi Keitaro
Publication year - 1990
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19900420308
Subject(s) - glucose oxidase , substrate (aquarium) , enzyme , amylase , chemistry , peroxidase , chromatography , hydrolysis , oxidase test , alpha amylase , biochemistry , biology , ecology
Using maltohexaose G 6 as a substrate, a convenient analytical method for amylase‐catalyzed hydrolytic reaction was developed by application of an enzymatic assay‐kit (glucose oxidase‐peroxidase) to measurement of glucose, produced on the reaction: G 6 →G 5 + G. The initial velocity was confirmed to be proportional to the initial concentration of amylase, examined, ∼ 100 μm. The new method was found in a good correlation with the Nelson and Somogyi method. Rate assay was also examined: The initial velocity is proportional to the enzyme concentration, ∼ 30 μm. Hence, this enzymatic assay method is recognized to be convenient and useful for the measurement of α‐amylase.