Effects of Aliphatic Alcohols on Formation of Cyclodextrin from Soluble Starch by Bacillus macerans Cyclodextrin Glucanotransferase

Effects of aliphatic alcohols such as ethanol, n‐butanol and n‐decanol on the formation of cyclodextrin (CD) from soluble starch by Bacillus macerans cyclodextrin glucanotransferase (CGTase) were experimentally studied at 50°C and pH 6.0. The addition of such aliphatic alcohols caused an increase in α‐CD yield, which extent was remarkable in n‐butanol. From the incubation of α‐CD with CGTase, such an increase was found to be due to a restriction on further transglycosylation of α‐CD into β‐CD by CGTase imposed by the aliphatic alcohols.