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The Effect of Substrate Diffusion Factor on Immobilized α‐Amylase
Author(s) -
Tarhan L.
Publication year - 1989
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19890410808
Subject(s) - glutaraldehyde , hexamethylenediamine , substrate (aquarium) , diffusion , chemistry , amylase , bacillus subtilis , acrolein , immobilized enzyme , polymer chemistry , chemical engineering , chromatography , enzyme , biochemistry , catalysis , biology , thermodynamics , ecology , polyamide , genetics , bacteria , engineering , physics
α‐Amylase of Bacillus subtilis was immobilized on acrolein‐styren copolymers of spongy and non‐spongy structures which had been modified with hexamethylenediamine followed by glutaraldehyde. Optimum bonding rates were determined from the immobilization experiments carried out with different amounts of α‐amylase, pH‐activity relationships, variation of reaction rates with the time due to the substrate diffusion limitation factor and the observed K m values were evaluated.