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GPC Separation and Identification of Starch‐Related Oligosaccharides Containing more than one Type of Glycosidic Linkage
Author(s) -
Kennedy J. F.,
Stevenson D. L.,
White C. A.
Publication year - 1989
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19890410209
Subject(s) - glycosidic bond , chemistry , hydrolysate , pullulan , chromatography , elution , polysaccharide , gel permeation chromatography , size exclusion chromatography , starch , hydrolysis , organic chemistry , enzyme , polymer
Gel permeation chromatography on Bio‐Gel P2 (‐400 mesh) has been used successfully to identify the structures and separate oligosaccharides of up to DP4 in the hydrolysates of pullulan, elsinan and nigeran solely on a size exclusion principle brought about by the secondary and tertiary structure of oligosaccharides containing mixed glycosidic linkages. The technique could not be applied to lichenan hydrolysates since the influence of (1 → 3)‐β‐ and (1 → 4)‐β‐D‐glycosidic linkages on the elution behaviour of oligosaccharides is very similar. However, results obtained for elsinan hydrolysates are in accordance with those obtained after exhaustive methylation and enzymatic analysis which required many work ups and fractionations on both paper and activated charcoal columns. Therefore, the use of Bio‐Gel P2 for structural analysis of oligosaccharides is a considerably valuable time saving tool in polysaccharide structural analysis providing the nature of the glycosidic linkages within the polysaccharide show different elution behaviour.