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Production of a Novel Inulin Fructotransferase (DFAI producing) by Arthrobacter globiformis S14‐3
Author(s) -
Seki K.,
Haraguchi K.,
Kishimoto M.,
Kobayashi S.,
Kainuma K.
Publication year - 1988
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19880401110
Subject(s) - inulin , arthrobacter , microorganism , chemistry , enzyme , enzyme assay , food science , biochemistry , bacteria , biology , genetics
A microorganism producing a novel inulin fructotransferase was isolated from a soil sample. The enzyme converted inulin into di‐D‐fructofuranose 1,2':2,1' dianhydride (DFAI) and a small amount of oligosaccharides. Through taxonomical studies, the microorganism was identified as Arthrobacter globiformis S14‐3. The method for preparation of DFAI from inulin was investigated and established. The enzyme showed maximal activity at pH 6.0 and 40°C. The enzyme was stable up to 70°C at pH 6.0.