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A Histological and Histochemical Study of Starch Granule Degradation in the Cotyledons of Lens culinaris
Author(s) -
Revilla M. A.,
Fernández Tárrago J.
Publication year - 1988
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19880400805
Subject(s) - cotyledon , starch , granule (geology) , amylase , degradation (telecommunications) , germination , chemistry , biochemistry , biology , botany , enzyme , paleontology , telecommunications , computer science
The pattern of starch granules degradation and amylase development in cotyledons of Lens culinaris Medik var. Castellana, was investigated by means of fluorescence microscopy and starch film method, respectively. Starch granules degradation in lentil cotyledons during germination started at the peripheral abaxial side and advanced towards the internal adaxial side of the cotyledon. However, amylase activity development took place from the whole periphery towards the center of the cotyledon. These results show the absence of a relationship between the zone of amylase activity and the degradation of starch granules. The absence of an embryonic axis did not affect the location of amylase activity but, on the other hand, did hinder the degradation of starch.