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Crosslinked Starch Entrapped in Agarose‐Beads. An Affinity Matrix for the Preparation of α‐D‐Mannose/α‐D‐Glucose Specific Lectins
Author(s) -
Sawhney S. Y.,
Nandedkar U. N.,
Bhide S. V.,
Kale N. R.
Publication year - 1988
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19880400704
Subject(s) - agarose , concanavalin a , starch , papain , chemistry , lectin , affinity chromatography , chromatography , sephadex , mannose , biochemistry , sepharose , matrix (chemical analysis) , polysaccharide , enzyme , in vitro
A simple biospecific affinity matrix like crosslinked starch entrapped in agarose‐beads is used for the isolation of α‐D‐mannose/α‐D‐glucose specific lectins such as Concanavalin A. pea and lentil. The adsorption capacities of these lectins for crosslinked starch are compared with those of Sephadex G‐50. The purified lectins were found to be identical to those obtained by using Sephadex G‐50 as an affinity matrix, as judged from their electrophoretic patterns (PAGE ‐ pH 4.5, SDS‐PAGE) and hemagglutination of papain treated rabbit erythrocytes.