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Studies on Purification and Kinetics of Glucose Isomerase on Hydrolysates of Starch
Author(s) -
ElSahy K. M.,
Siliha H. A.,
Fahmy A. H.
Publication year - 1987
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19870390209
Subject(s) - amberlite , chemistry , chromatography , acetone , ammonium , enzyme , starch , hydrolysate , xylose isomerase , fermentation , elution , biochemistry , adsorption , isomerase , hydrolysis , organic chemistry
Glucose isomerase was produced from Streptomyces phaeochromogenes by aerobic fermentation at 28°C for 24 h. The crude enzyme was obtained by disintegrating the harvested cells. Ammonium sulphate at a saturation of 0.65 gave the maximum enzyme recovery 89.4%) from the crude extract, while acetone gave 66.4% at a concentration of 3/1 (v/v). The crude enzyme extract was purified by concentration, dialysis, precipitation with ammonium sulphate, passing through a column of Amberlite, and then the eluate was treated with acetone to precipitate the enzyme. The kinetic behavior was studied and it was found that optimum D ‐glucose concentration was 0.8‐ M . No change in K m value was observed, while V max was affected.