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Effect of the Reversion of Aspergillus niger Auxotrophic Mutants to Prototrophic Forms on their Glucoamylolytic Activity
Author(s) -
Ilczuk Z.,
Fiedurek J.
Publication year - 1986
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19860380807
Subject(s) - auxotrophy , aspergillus niger , reversion , mutant , biology , enzyme , biochemistry , gene , phenotype
Abstract Prototrophic revertants were obtained from 4 auxotrophic mutants A. niger , 2 of which originated from amylolytically highly active prototroph A. niger C, whereas 2 from weakly active prototrophs A. niger 23 and 71. A hundred of revertants randomly chosen from each group were initially evaluated with respect to total amylolytic activity. Ten revertants which in this respect reached the highest indices were closely studied in regard to the synthesis of glucoamylase. All of them showed a distinct increase in the activity of this enzyme in relation to their ancestor both auxotrophic and prototrophic. In the case of revertants, derivatives of A. niger C prototroph, maximal glucoamylase activity increased over 40%. Among the derivatives of A. niger 71 prototroph the increase in glucoamylase activity exceeded many times the level of glucoamylase activity of its prototrophic ancestor.