Premium
Further Methods for the Characterization of Natural Amyloses as Potential Substrates for the Assay of α‐Amylase
Author(s) -
Kennedy J. F.,
White C. A.,
Stevenson D. L.,
Gutlin K.
Publication year - 1986
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19860380306
Subject(s) - chemistry , gel permeation chromatography , amylose , chromatography , hydrolysate , amylase , glucan , permeation , oligosaccharide , enzyme , biochemistry , starch , hydrolysis , organic chemistry , membrane , polymer
Abstract Improved methods have been devised for the assessment of amylose samples and their suitability for conversion by dyeing with Cibacron Blue 3G‐A to give insoluble coloured substrates for the assay of α‐amylase. The new methods reported herein are based on: the determination of oligosaccharide component composition of water soluble fractions and enzymatic hydrolysates using gel permeation chromatography with Bio‐Gel P‐2 media; gel permeation chromatography to assess molecular weight distributions; and enzymic degradation using α‐amylase (1,4‐α‐ D ‐glucan glucanohydrolase, EC 3.2.1.1) and β‐amylase (1,4‐α‐ D ‐glucan maltohydrolase, EC 3.2.1.2).