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A Polarographic Cyclodextrin Assay Based on Linoleate‐Cyclodextrin Complex Formation in a Lipoxygenase Reaction
Author(s) -
Laakso S.,
Leivo P.,
Mäkelä M.,
Korpela T.
Publication year - 1984
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19840361208
Subject(s) - chemistry , cyclodextrin , lipoxygenase , dextrin , chromatography , starch , polarography , linoleic acid , enzyme , organic chemistry , inorganic chemistry , fatty acid
A polarographic assay of cyclodextrins in turbid dextrin mixtures is described. The method is based on the ability of cyclodextrins to form complexes with linoleic acid and on the inability of soybean lipoxygenase to oxidize the complexed acid. The degree of complexation is measured with a simple Clark's electrode as a reduced rate of oxygenation of linoleate. Cyclodextrin concentrations down to 0.02–0.04‐mM can be assayed. The method is rapid to carry out because several successive samples can be added into a single lipoxygenase assay mixture. The method was applied to analyses of immobilized cyclodextrins as well as cyclodextrins in complex mixtures of starch and starch degrading enzymes. The conditions for spectrophotometric measurement of the reaction are also described.