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Purification of a Bacillus thermoglucosidius Exo‐oligo‐1, 6‐glucosidase by a Simple and Rapid Four‐step Method
Author(s) -
Suzuki Y.,
Kano Y.,
Nishimura N.
Publication year - 1984
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19840360806
Subject(s) - thermophile , chemistry , chromatography , hydrolysis , dextrin , enzyme , yield (engineering) , homogeneous , bacillus (shape) , methionine , amino acid , biochemistry , biology , microbiology and biotechnology , materials science , physics , starch , metallurgy , thermodynamics
A p‐nitrophenyl‐α‐D‐glucopyranoside‐hydrolyzing exo‐oligo‐ 1,6‐glucosidase (dextrin 6‐α‐glucanohydrolase, EC. 3.2.1.10) of a thermophile Bacillus thermoglucosidius was purified to an electrophoretically homogeneous form with a yield of 47% by a rapid and simple four‐step procedure including the enzyme desorption from immunoadsorbents by 3‐M NaSCN. Its specific activity was 421 μmol p‐nitrophenyl‐α‐D‐glucopyranoside hydrolyzed/min/mg protein at 60° C and pH 6.8. The aminoterminal amino acid of the enzyme was determined as methionine.