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Intensification of Amylase Synthesis with Aspergillus niger by Way of Multistage Mutagenization
Author(s) -
Ilczuk Z.,
Fiedurek J.,
Paszczynski A.
Publication year - 1983
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19830351107
Subject(s) - aspergillus niger , amylase , chemistry , strain (injury) , aspergillus , irradiation , ethyleneimine , conidium , microbiology and biotechnology , food science , biology , enzyme , biochemistry , botany , organic chemistry , anatomy , polymer , physics , nuclear physics
Conidia of amylolytically active Aspergillus niger C strain were subjected to four‐stage mutagenization, using different combinations of mutagens in each stage (stage I – UV irradiation + ethyleneimine, stage II – UV irradiation + nitrosomethylurea, stage III – UV irradiation + N‐methyl‐N′‐nitro‐N‐nitrosoguanidine, stage IV – acryflavine). In all, 378 strains were isolated after mutagenization, which were initially evaluated for the total amylolytic activity by the method of test‐tube microculture. Nine most active mutants were then tested in submerged culture determining the activity of glucoamylase and α‐amylase in post‐culture liquid. The activity of α‐amylase increased from over 7 to nearly 31%, and that of glucoamylase from 4 to over 61%.