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An Improved Productivity and Stability Test for Immobilized Enzymes with Reference to Glucose Isomerase
Author(s) -
van Tilburg R.,
Roels J. A.
Publication year - 1982
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19820340409
Subject(s) - chemistry , glucose 6 phosphate isomerase , productivity , isomerase , fructose , enzyme , chromatography , biochemistry , economics , macroeconomics
Commercial preparations of immobilized glucose isomerase have a long productivity half life at normal operating temperatures, i. e. about 50–60 days. Hence, the laboratory assay for the productivity and stability of the product is rather time consuming. The present publication describes a quick test which is carried out at 70°C within 7 days. In this procedure the known temperature dependences of the deactivation rate of the enzyme preparation, of its activity and of the equilibrium constant of the transformation of glucose to fructose are used. Extensive experimental evidence concerning the reliability of the test for the prediction of the behaviour of the product at 60°C is provided. The effect of particle size on initial flow velocity, half life and productivity are discussed. The improved productivity and stability test is a valuable tool to the testing of immobilized glucose isomerase and other immobilized enzymes.