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Determination of Amylose: Amylopectin Ratios of Starches
Author(s) -
Sargeant J. G.
Publication year - 1982
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19820340306
Subject(s) - amylopectin , amylose , isoamylase , chemistry , chromatography , gel permeation chromatography , starch , organic chemistry , polymer , amylase , enzyme
A method has been developed for the determination of amylose: amylopectin ratios of starches which offers an alternative to iodimetric methods. Starches solubilized in dimethylsulphoxide were debranched with isoamylase and the resulting linear components quantitated by gel permeation chromatography on a column of Sepharose CL‐6B.