Immobilization of Glucose Isomerase on Radiation‐Modified Gelatine Gel | Zendy

Bachman S. | Zendy; Gebicka L. | Zendy; Gasyna Z. | Zendy

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Immobilization of Glucose Isomerase on Radiation‐Modified Gelatine Gel

Author(s) -

Bachman S.,

Gebicka L.,

Gasyna Z.

Publication year - 1981

Publication title -

starch ‐ stärke

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.62

H-Index - 82

eISSN - 1521-379X

pISSN - 0038-9056

DOI - 10.1002/star.19810330208

Subject(s) - glutaraldehyde , glucose 6 phosphate isomerase , enzyme , chemistry , isomerase , isomerization , immobilized enzyme , chromatography , specific activity , biochemistry , catalysis

Whole‐cell glucose isomerase immobilization on radiation‐modified gelatine gel was demonstrated. The enzyme was stabilized within the cells by heat‐treatment at 70°C. The heat‐treated cells were mixed with radiation‐modified gelatine gel and cross‐linked by glutaraldehyde. Immobilized enzyme exhibited 70–75% activity as compared to the free cells. Several properties of immobilized glucose isomerase were studied under various reaction conditions. A continuous isomerization was performed using a column packed with cell granules. It has been found, that the half‐life of the immobilized glucose isomerase amounted to 650 h at 60°C.

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