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Biotechnical Problems of Isoglucose Production. Part 4. Production and Characterization of immobilized Glucoamylase
Author(s) -
Holló J.,
László E.,
Hoschke A.
Publication year - 1981
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19810330205
Subject(s) - glutaraldehyde , cellulose , amine gas treating , chemistry , starch , reagent , hydrolysis , aspergillus niger , immobilized enzyme , enzyme , organic chemistry , biochemistry , chromatography
Of the up‐to‐date industrial technologies applied in the starch industry, saccharification is practically the only procedure carried out batchwise with dissolved enzyme. No inexpensive industrial scale Technology has been developed so far for immobilization of enzyme. Therefore, cellulose amine and DEAE‐cellulose were investigated with respect to their application as carriers. For immobilization industrial Aspergillus niger glucoamylase (Miles L‐100) was used and glutaraldehyde and/or p‐benzochinone as binding reagent. In the case of cellulose amine carrier, most favourable binding can be attained with smallest particle‐size. With DEAE‐cellulose, the amount of enzymes bound depends on the swelling of the carrier, and binding can be observed even in the pores of DEAE‐cellulose.