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Determination of Amylase Activity in Corn Using a Chromogenic Substrate
Author(s) -
PárkányGyárfás A.,
VámosVigyázó L.
Publication year - 1979
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19790311003
Subject(s) - amylase , substrate (aquarium) , chromogenic , chemistry , chromatography , volume (thermodynamics) , substrate specificity , enzyme , biochemistry , biology , ecology , physics , quantum mechanics
Amylase activity in corn was determined with the Phadebas Amylase Test. Substrate concentration was varied between 1 and 6 tablets in a reaction volume of 10 ml. Reaction rate varied with substrate concentration according to a sigmoidal curve. The apparent kinetic parameters V max and K m were established. In the reaction volume of 10 ml 2 tablets of the Phadebas Amylase Test yielded a linear relationship between reaction rate and concentration of the enzyme extract. The substrate was attacked by a β‐amylase enzyme preparation as well. Amylase activity was found to be highest at 50 °C.
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