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Simplified Determination of Amylose in Milled Rice
Author(s) -
Sowbhagya C. M.,
Bhattacharya K. R.
Publication year - 1979
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19790310506
Subject(s) - defatting , amylose , chemistry , petroleum ether , chromatography , aqueous solution , rice flour , extraction (chemistry) , organic chemistry , starch , raw material
Previous defatting is essential for correct determination of amylose content in rice by colorimetric iodine method. Since no simplified method of defatting of flour is satisfactory, a simple method involving defatting in liquid phase has been developed. The flour is dispersed in diluted alkali, and the dispersion is shaken successively with petroleum ether and carbon tetrachloride. The aqueous solution is pipetted from top for colour development. The method gives results essentially identical to that given by defatted flour. The colour is developed and read at about pH 7.5 and at 630 nm.