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Immobilization of Glucose Isomerase in Microbial Cells. Part 2. Properties of Immobilized Glucose Isomerase in Cell Granule Preparations
Author(s) -
Kasumi T.,
Tsumura N.,
Kobayashi T.
Publication year - 1979
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19790310107
Subject(s) - glucose 6 phosphate isomerase , immobilized enzyme , enzyme , chemistry , isomerase , granule (geology) , enzyme assay , biochemistry , chromatography , kinetics , biology , physics , quantum mechanics , paleontology
Some properties of immobilized glucose isomerase in whole cell preparations were investigated. Immobilized glucose isomerase showed a similar velocity pattern to the native free enzyme. But the reaction system of immobilized enzyme was thought to be influenced by diffusion. The immobilized enzyme was much superior to the free enzyme in heat and pH stability and resistance to the inhibitory effect of several metal ions. In continuous process immobilized glucose isomerase showed a considerable durability. Decrease of activity of the immobilized enzyme column was approximately expressed by an exponential function of time.