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Immobilization of Glucose Isomerase in Microbial Cells. Part 1. Preparation of Cell Granules and their Enzyme Retentivities
Author(s) -
Tsumura N.,
Kasumi T.,
Ishikawa M.
Publication year - 1978
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19780301205
Subject(s) - glutaraldehyde , glucose 6 phosphate isomerase , granule (geology) , chemistry , reagent , enzyme , biochemistry , gelatin , bifunctional , cell encapsulation , enzyme assay , cell , chromatography , organic chemistry , catalysis , biology , paleontology
Abstract An immobilization method for glucose isomerase in microbial cells was demonstrated, in which cells derived from Streptomyces sp. were aggregated with chitosan and cross‐linked with bifunctional reagents. Enzyme retentivity of the product was investigated in relation to the enzyme activity change during cell treatment. The reaction conditions with glutaraldehyde, i.e. the reagent concentration, reaction time and pH of the reaction solution were examined. The effect of gelatin glue addition to cell granule formation on the binding toughness of the product was also investigated. Swelling capacity of the granule was observed to be restrained by cross‐linking. A continuous isomerization performance using a colum packed with cell granules was carried out.