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Immobilisation of α‐Amylase on Polyaromatic and Titanium Compounds Incorporating a Magnetic Material
Author(s) -
Kennedy J. F.,
Barker S. A.,
White C. A.
Publication year - 1977
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19770290707
Subject(s) - thermostability , titanium , chemistry , titanium oxide , oxide , immobilized enzyme , amylase , nuclear chemistry , enzyme , chemical engineering , inorganic chemistry , organic chemistry , engineering
Three methods of preparing immobilised α‐amylase on supports which incorporate magnetic properties have been developed. The use of diazotized 1,3‐diaminobenzene coated on magnetic iron oxide, which was itself either uncoated or coated with hydrous titanium (IV) oxide, was found to result in higher bound activities of the immobilised enzyme. The immobilised enzyme prepared from diazotized 1,3‐diaminobenzene coated magnetic iron oxide support showed excellent stability towards freeze drying. A preparation of immobilised enzyme using magnetic iron coated with hydrous titanium (IV) oxide resulted in a greater thermostability. This support can be prepared in situ, overcoming the problem of loss of activity of the freeze dried immobilised enzyme.