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Multiplicity of Glucoamylase of Aspergillus oryzae Part 1. Separation and Purification of Three Forms of Glucoamylase
Author(s) -
Miah M. N. N.,
Ueda S.
Publication year - 1977
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19770290604
Subject(s) - sephadex , chromatography , aspergillus oryzae , size exclusion chromatography , chemistry , isoelectric focusing , polyacrylamide gel electrophoresis , bran , ethanol precipitation , homogeneous , ion chromatography , isoelectric point , biochemistry , enzyme , extraction (chemistry) , raw material , organic chemistry , physics , thermodynamics
The glucoamylase system of Aspergillus oryzae cultured on wheat bran was separated into 3 active fractions by (NH 4 ) 2 SO 4 , rivanol and ethanol precipitation followed by ion exchange chromatography on DEAE‐Sephadex A‐25. One of these fractions, referred to as glucoamylase I was purified by further chromatography on hydroxyapatite gel and Sephadex G‐200. The other two fractions referred to as glucoamylase II and III were purified by further gel filtration on Sephadex G‐200. The purified glucoamylases were found to be homogeneous on 7.5% polyacrylamide gel electrophoresis and isoelectric focusing by carrier ampholites.