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The Action of alpha‐Amylase on Dyed and Natural Amyloses
Author(s) -
Kennedy J. F.
Publication year - 1977
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19770290403
Subject(s) - amylose , chemistry , chromatography , amylase , hydrolysis , analyser , alpha amylase , biochemistry , enzyme , starch
The products of the action of α‐amylase upon Amylose‐Cibacron Blue 3G‐A, a dyed form of amylose in current use for the assay of α‐amylase, have been examined by gel permeation chromatography and colorimetric assay using a converted Jeol JLC—6AH carbohydrate analyser. Comparison of the product profiles with those obtained for the hydrolysis of the parent amylose under identical conditions indicated a modified action pattern of the α‐amylase upon the dyed amylose. These observations are discussed with particular reference to the use of Amylose‐Cibacron Blue 3G‐A for assay of α‐amylase in clinical specimens.