Premium
The Characterisation of Starch and Its Components Part 4. The Specific Estimation of Glucose Using Glucose Oxidase
Author(s) -
Banks W.,
Greenwood C. T.
Publication year - 1971
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/star.19710230703
Subject(s) - glucose oxidase , chemistry , glycerol , starch , aqueous solution , peroxidase , absorbance , chromatography , incubation , oxidase test , enzyme , biochemistry , organic chemistry
The glucose oxidase/peroxidase technique for determining microgram quantities of glucose in aqueous solution has been critically examined. The function of each of the constituents in the solution has been studied, and the relation between time and temperature of incubation determined. Potential errors due to the temperature‐dependent pH‐behaviour of tris buffers, and the presence in the solution to be analyzed of high concentrations of buffer salts, glycerol, and reducing substances, are discussed. The relation between the absorbance, and the type and normality of acid used to stabilize the colour is examined.