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Estimating the proportions in a mixed sample using transcriptomics
Author(s) -
Clarke Bertrand,
Clarke Jennifer
Publication year - 2014
Publication title -
stat
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.61
H-Index - 18
ISSN - 2049-1573
DOI - 10.1002/sta4.65
Subject(s) - stromal cell , tissue sample , sample (material) , transcriptome , gene expression , biology , cancer cell , gene , cancer research , computational biology , cancer , chemistry , genetics , anatomy , chromatography
Often, biomedical researchers take a tissue sample from a tumour that unintentionally contains tumour cells and stromal cells so that gene expression from the sample represents two distinct kinds of cells. This is tolerated because techniques for separating tumour and stromal cells are expensive and time‐consuming and the act of separation can alter gene expression. So, it is desirable to have a technique for estimating the proportion of tumour cells in a mixed sample to improve detection of differential expression in cancer cells. © 2014 The Authors. Stat Published by John Wiley & Sons Ltd.