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Preparation of open‐tubular CEC column bonded with 6‐O‐monotosyl‐deoxy‐β‐cyclodextrin and its application in the enantioseparation of several related benzimidazoles
Author(s) -
Fan Shitong,
Guan Jin,
Yan Feng,
Zhang Dongxiang,
Shi Shuang,
Wang Silin
Publication year - 2020
Publication title -
separation science plus
Language(s) - English
Resource type - Journals
ISSN - 2573-1815
DOI - 10.1002/sscp.202000014
Subject(s) - capillary electrochromatography , chromatography , analyte , monolithic hplc column , chemistry , enantiomer , cyclodextrin , electrochromatography , resolution (logic) , capillary action , theoretical plate , high performance liquid chromatography , analytical chemistry (journal) , capillary electrophoresis , materials science , stereochemistry , artificial intelligence , computer science , composite material
A new chiral open‐tubular capillary electrochromatography column was prepared with 6‐O‐monotosyl‐deoxy‐β‐cyclodextrin as staionary phase by covalent immobilization on inner wall of a fused‐silica capillary. Preparation procedure of the open‐tubular column was investigated and resulting surface morphology was determined using scanning electron microscopy. Moreover, the enantiomeric separation performance of open‐tubular capillary electrochromatography column was evaluated by using several related benzimidazoles as model analytes, including pantoprazole, tenatoprazole, omeprazole, and lansoprazole. The primary factors affecting its separation efficiency, such as type, concentration, and pH of buffer, applied voltage were optimized. In the optimum conditions, the enantiomers of four analytes revealed high resolutions of 5.75–8.83. The relative standard deviation values for the migrated times and resolutions of the model analyte representing intraday, interday, and column‐to‐column were less than 1.8 and 1.3%, respectively. The chiral open‐tubular capillary electrochromatography column still had satisfactory separation performance after continuous use for 72 h or intermittent use for 3 months.