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Simultaneous quantification of perindopril erbumine, indapamide and amlodipine besylate in newer combination of anti‐hypertensive drugs in pharmaceutical dosage form by thin layer chromatography method
Author(s) -
Patel Kirtan,
Chhalotiya Usmangani,
Kachhiya Hetaben,
Patel Jay,
Shah Dimal,
Nagda Dhruti
Publication year - 2020
Publication title -
separation science plus
Language(s) - English
Resource type - Journals
ISSN - 2573-1815
DOI - 10.1002/sscp.202000010
Subject(s) - indapamide , amlodipine , perindopril , chromatography , dosage form , chemistry , materials science , pharmacology , medicine , diuretic , blood pressure
A sensitive, precise, robust and accurate high performance thin layer chromatographic method was developed and validated base on ICH Q2 (R1) guideline for the simultaneous estimation of Perindopril erbumine, Indapamide and Amlodipine besylate in bulk and combined Triplixam marketed tablet dosage formulation. Pre‐coated silica gel aluminum plate 60 F 254 was selected as the stationary phase and dichloromethane: methanol: toluene: glacial acetic acid (7: 1: 2: 0.1, v/v/v/v) was used as developing mobile phase. The common detection wavelength for Perindopril erbumine, Indapamide and Amlodipine besylate was carried out at 215 nm. The method was validated for linearity, precision, accuracy, and robustness, limit of detection and limit of quantitation as per ICH parameters. The correlation coefficient (r 2 ) of Perindopril erbumine, Indapamide and Amlodipine besylate were found to be 0.9987, 0.9987 and 0.9996, respectively. The mean of percentage recovery of Perindopril erbumine, Indapamide and Amlodipine besylate were found to be 99.38‐101.81, 100.61‐102.68 and 99.87‐101.95%, respectively. The proposed thin layer chromatography method has potential qualitative as well as quantitative applications for simultaneous estimation of Perindopril erbumine, Indapamide and amlodipine besylate in bulk and pharmaceutical dosage form.