Premium
Productivity of a preparative high‐performance liquid chromatography isolation of anacardic acids from cashew nut shell liquid
Author(s) -
Oiram Filho Francisco,
Zocolo Guilherme J.,
Canuto Kirley M.,
Silva Junior Ivanildo J. da,
Brito Edy S.
Publication year - 2019
Publication title -
separation science plus
Language(s) - English
Resource type - Journals
ISSN - 2573-1815
DOI - 10.1002/sscp.201900014
Subject(s) - cashew nut , chemistry , chromatography , nut , high performance liquid chromatography , acetic acid , shell (structure) , solvent , yield (engineering) , alkyl , organic chemistry , food science , materials science , structural engineering , engineering , metallurgy , composite material
Abstract The anacardic acids are alkyl phenols, with biological activities, obtained as a byproduct from cashew nut processing. The anacardic acids isolation by preparative reversed phase high‐performance liquid chromatography was developed injecting large pulse from 50 to 200 mg of cashew nut shell liquid through a C 18 column eluted with methanol, water and acetic acid (80:20:1). The separations were carried out at 25°C and monitored at 280 nm. Yield, recovery, purity, solvent consumption and productivity were analyzed. The results of anacardic acids isolation pointed the concentration loading of 100 mg as a process threshold leading to a productivity of 0.681 Kg.day −1 .Kg −1 of anacardic acids with purity of 96.41, 95.32 and 90.77% for anacardic acid triene, diene and monoene, respectively.