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Structural characterization and quantitative determination of bioactive compounds in ethanolic extracts of Boerhaavia diffusa L. by liquid chromatography with tandem mass spectrometry
Author(s) -
Kumar Sunil,
Singh Awantika,
Singh Bikarma,
Maurya Rakesh,
Kumar Brijesh
Publication year - 2018
Publication title -
separation science plus
Language(s) - English
Resource type - Journals
ISSN - 2573-1815
DOI - 10.1002/sscp.201800056
Subject(s) - chromatography , chemistry , electrospray ionization , repeatability , detection limit , mass spectrometry , tandem mass spectrometry , high performance liquid chromatography , liquid chromatography–mass spectrometry , relative standard deviation , tandem , electrospray , analytical chemistry (journal) , materials science , composite material
Rapid and sensitive liquid chromatography with tandem mass spectrometry methods were developed for structural characterization and quantitative determination of bioactive compounds in Boerhaavia diffusa using high‐performance liquid chromatography with electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry and ultra high performance liquid chromatography with electrospray ionization hybrid triple quadrupole‐linear ion trap tandem mass spectrometry in negative ionization mode. Fifty seven compounds were identified and characterized in ethanolic extracts of root, stem and leaf of B. diffusa . Ten compounds that include characteristic compounds eupalitin‐3 ‐O ‐galactopyranoside, eupalitin, and boeravinone B were successfully separated on an Acquity UPLC BEH™ C 18 column (1.7 μm, 2.1 mm × 50 mm) within 6 min. The validation parameters showed satisfactory linearities (R 2 ≥ 0.9991), accuracy (limit of detection: 0.01–1.40 ng mL −1 and limit of quantitation: 0.05–4.26 ng mL −1 ) and precision (% relative standard deviation: 0.25–3.45 for intra‐day, and 0.26–3.25 for inter‐day), stability (% relative standard deviation: 0.16–1.87) and recoveries with relative standard deviation (98.67–104.16, % relative standard deviation: 0.20–4.06). Eupalitin‐3 ‐O ‐galactopyranoside (18530.00 μg g −1 ) was one of the most abundant among the quantified compounds followed by eupalitin (1258.00 μg g −1 ) in leaf of Samba district. The developed methods could be effectively used for quality control of B. diffusa due to its accuracy, stability, and repeatability.