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Validated HPLC Method for the simultaneous determination of acyclovir and co‐administered vitamin B 3 and gabapentin in spiked human plasma
Author(s) -
Sayed Rania,
ElMasry Manal,
Hassan Wafaa,
ElMammli Magda,
Shalaby Abdalla,
AboulEnein Hassan Y.
Publication year - 2018
Publication title -
separation science plus
Language(s) - English
Resource type - Journals
ISSN - 2573-1815
DOI - 10.1002/sscp.201800040
Subject(s) - chromatography , methanol , high performance liquid chromatography , gabapentin , formic acid , protein precipitation , chemistry , vitamin , potassium , elution , medicine , organic chemistry , biochemistry , alternative medicine , pathology
In this work, a new, sensitive and accurate high‐performance liquid chromatographic method for the simultaneous determination of acyclovir and co‐administered vitamin B 3 and gabapentin used in treatment of herpes zoster disease was developed and validated. Protein precipitation with methanol was performed to extract the cited drugs from plasma. Thermo Hypersil (250 mm × 4.6 mm I.D, 5 μm particle size) column was used for the chromatographic separation. Isocratic elution of mobile phase consisting of methanol and 0.03 M potassium dihydrogen orthophosphate adjusted to pH 3.5 with 0.1% orthophosphoric acid (30:70, v/v) was used for separation. Photodiode array detector was used and detection was performed at 210 nm. The method was linear over the concentration range 1–100 μg/mL for both acyclovir and vitamin B 3 and 5–150 μg/mL for gabapentin. The proposed method was validated and successfully applied for the commercial dosage forms of the three drugs and in human plasma. Statistical analysis of the results revealed its high accuracy and good precision.