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Purification and activity research of hypoglycemic components from the extract of mulberry ( Morus alba L.) leaves
Author(s) -
Han Tian,
Wang Wei,
Cao Xueli
Publication year - 2018
Publication title -
separation science plus
Language(s) - English
Resource type - Journals
ISSN - 2573-1815
DOI - 10.1002/sscp.201800036
Subject(s) - chemistry , sucrase , chromatography , electrospray ionization , mass spectrometry , alpha glucosidase , maltase , biochemistry , enzyme
Mulberry leaf is a traditional therapy for diabetes. A simple ion‐exchange resin column chromatography method to separate active hypoglycemic components from the extract of Morus alba L. leaves was investigated in this study. The resins (001 × 7, HD‐2 and HZ202) used in this study were common and economical. Five pure compounds including myo‐inositol ( 1 ), 1‐deoxynojirimycin ( 2 ), 2‐ O ‐α‐ d ‐galactopyranosyl‐1‐deoxynojirimycin ( 3 ), fagomine ( 4 ), and 1,4‐dideoxy‐1,4‐imino‐(2‐ O ‐β‐ d ‐glucopyranosyl)‐ d ‐arabinitol ( 5 ) were obtained. The structures of the obtained components were identified by electrospray ionization mass spectrometry and NMR spectroscopy. The results of an in vitro assay indicated that all of the obtained compounds exhibited significant α‐glucosidase inhibitory activities. Among of the five obtained compounds, 1‐deoxynojirimycin showed the strongest inhibitory activity against both sucrase and maltase.