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New stationary phase for capillary liquid chromatography based on polydimethylsiloxane immobilized onto a Monolithic Silica Support
Author(s) -
Pires Fabiane,
Otálora Julie Alexandra Bautista,
Bottoli Carla Beatriz Grespan
Publication year - 2018
Publication title -
separation science plus
Language(s) - English
Resource type - Journals
ISSN - 2573-1815
DOI - 10.1002/sscp.201800033
Subject(s) - polydimethylsiloxane , methyltrimethoxysilane , monolith , chromatography , capillary action , alkylbenzenes , monolithic hplc column , materials science , capillary electrochromatography , high performance liquid chromatography , chemistry , stationary phase , nanotechnology , organic chemistry , composite material , coating , catalysis , benzene
A new stationary phase based on polydimethylsiloxane immobilized onto a monolithic silica support was prepared for performing reversed‐phase capillary liquid chromatography. Silica monoliths, using tetramethoxysilane as precursor, and hybrid monoliths, using tetramethoxysilane and methyltrimethoxysilane as precursors, were prepared by the sol–gel process. Polydimethylsiloxane was deposited on the monolithic matrix and its immobilization was performed through thermal treatment. Characterization was made by field‐emission scanning electron microscopy and by chromatographic evaluation for the separation of six alkylbenzenes. An efficiency of 79 500 plates/m was reached for columns of 200 μm id, demonstrating that it would be a promising alternative to a reversed‐phase column in capillary liquid chromatography.