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Polymer Coatings to Minimize Protein Adsorption in Solid‐State Nanopores
Author(s) -
Awasthi Saurabh,
Sriboonpeng Pongsatorn,
Ying Cuifeng,
Houghtaling Jared,
Shorubalko Ivan,
Marion Sanjin,
Davis Sebastian James,
Sola Laura,
Chiari Marcella,
Radenovic Aleksandra,
Mayer Michael
Publication year - 2020
Publication title -
small methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.66
H-Index - 46
ISSN - 2366-9608
DOI - 10.1002/smtd.202000177
Subject(s) - nanopore , polymer , biomolecule , nanotechnology , coating , materials science , solid state , solid surface , protein adsorption , membrane , adsorption , fabrication , chemistry , chemical physics , composite material , organic chemistry , medicine , biochemistry , alternative medicine , pathology
Nanopore‐based resistive‐pulse recordings represent a promising approach for single‐molecule biophysics with applications ranging from rapid DNA and RNA sequencing to “fingerprinting” proteins. Based on advances in fabrication methods, solid‐state nanopores are increasingly providing an alternative to proteinaceous nanopores from living organisms; their widespread adoption is, however, slowed by nonspecific interactions between biomolecules and pore walls, which can cause artifacts and pore clogging. Although efforts to minimize these interactions by tailoring surface chemistry using various physisorbed or chemisorbed coatings have made progress, a straightforward, robust, and effective coating method is needed to improve the robustness of nanopore recordings. Here, covalently attached nanopore surface coatings are prepared from three different polymers using a straightforward “dip and rinse” approach and compared to each other regarding their ability to minimize nonspecific interactions with proteins. It is demonstrated that polymer coatings approach the performance of fluid lipid coatings with respect to minimizing these interactions. Moreover, these polymer coatings enable accurate estimates of the volumes and spheroidal shapes of freely translocating proteins; uncoated or inadequately coated solid‐state pores do not have this capability. In addition, these polymer coatings impart physical and chemical stability and enable efficient and label‐free characterization of single proteins without requiring harsh cleaning protocols between experiments.