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Recognition of Single‐Point Mutation Using a Biological Nanopore
Author(s) -
Liu Ping,
Kawano Ryuji
Publication year - 2020
Publication title -
small methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.66
H-Index - 46
ISSN - 2366-9608
DOI - 10.1002/smtd.202000101
Subject(s) - nanopore , point mutation , oligonucleotide , nanopore sequencing , chromosomal translocation , dna , mutation , computational biology , nanotechnology , genetics , biophysics , biology , gene , materials science , dna sequencing
Here the recognition of a single‐point mutation in oligonucleotides is described by using nanopore measurements. The translocation behavior of a series of mutated DNA strands, hybridized with a complementary DNA probe, is analyzed via blocking current and unzipping time. Discernment of the mutation position at the single nucleotide level is achieved by analysis of a 2D graph of the bootstrapped translocation data. The proposed approach provides a useful tool for the mutation detection of oligonucleotides secreted from tumor cells and is applicable in simple and label‐free diagnoses as a nanopore liquid biopsy.