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Single‐Molecule Switching: Fluorescence Polarization Control for On–Off Switching of Single Molecules at Cryogenic Temperatures (Small Methods 9/2018)
Author(s) -
Hulleman Christiaan N.,
Huisman Maximiliaan,
Moerland Robert J.,
Grünwald David,
Stallinga Sjoerd,
Rieger Bernd
Publication year - 2018
Publication title -
small methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.66
H-Index - 46
ISSN - 2366-9608
DOI - 10.1002/smtd.201870044
Subject(s) - dichroic glass , excitation , laser , polarization (electrochemistry) , optics , fluorescence , linear polarization , elliptical polarization , fluorescence anisotropy , dichroic filter , materials science , molecule , polarization rotator , physics , optoelectronics , chemistry , birefringence , quantum mechanics
In article number 1700323 , a sparsity‐inducing scheme based on widefield stimulated emission depletion, which requires stringent control of the polarization state of both the excitation and depletion laser, is introduced by Bernd Rieger and co‐workers for superresolution fluorescence microscopy. The ideal state involves excitation and depletion lasers with linear polarizations, orthogonally oriented in the sample plane. This state is obtained from linearly polarized light from the laser, which is made elliptically polarized before reflecting off the dichroic mirror.