Premium
Simultaneous Quantification and Visualization of Titanium Dioxide Nanomaterial Uptake at the Single Cell Level in an In Vitro Model of the Human Small Intestine
Author(s) -
Meyer Thomas,
Venus Tom,
Sieg Holger,
Böhmert Linda,
Kunz Birgitta M.,
Krause Benjamin,
Jalili Pegah,
Hogeveen Kevin,
Chevance Soizic,
Gauffre Fabienne,
Burel Agnes,
Jungnickel Harald,
Tentschert Jutta,
Laux Peter,
Luch Andreas,
Braeuning Albert,
Lampen Alfonso,
Fessard Valerie,
Meijer Jan,
EstrelaLopis Irina
Publication year - 2019
Publication title -
small methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.66
H-Index - 46
ISSN - 2366-9608
DOI - 10.1002/smtd.201800540
Subject(s) - nanomaterials , nanotechnology , chemistry , biophysics , nanoparticle , titanium dioxide , transmission electron microscopy , biomolecule , materials science , biology , metallurgy
Useful properties render titanium dioxide nanomaterials (NMs) to be one of the most commonly used NMs worldwide. TiO 2 powder is used as food additives (E171), which may contain up to 36% nanoparticles. Consequently, humans could be exposed to comparatively high amounts of NMs that may induce adverse effects of chronic exposure conditions. Visualization and quantification of cellular NM uptake as well as their interactions with biomolecules within cells are key issues regarding risk assessment. Advanced quantitative imaging tools for NM detection within biological environments are therefore required. A combination of the label‐free spatially resolved dosimetric tools, microresolved particle induced X‐ray emission and Rutherford backscattering, together with high resolution imaging techniques, such as time‐of‐flight secondary ion mass spectrometry and transmission electron microscopy, are applied to visualize the cellular translocation pattern of TiO 2 NMs and to quantify the NM‐load, cellular major, and trace elements in differentiated Caco‐2 cells as a function of their surface properties at the single cell level. Internalized NMs are not only able to impair the cellular homeostasis by themselves, but also to induce an intracellular redistribution of metabolically relevant elements such as phosphorus, sulfur, iron, and copper.