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Surface‐Enhanced Raman Nanoprobes with Embedded Standards for Quantitative Cholesterol Detection
Author(s) -
Jiang Xin,
Tan Ziyang,
Lin Li,
He Jing,
He Chang,
Thackray Benjamin D.,
Zhang Yuqing,
Ye Jian
Publication year - 2018
Publication title -
small methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.66
H-Index - 46
ISSN - 2366-9608
DOI - 10.1002/smtd.201800182
Subject(s) - raman scattering , reproducibility , detection limit , raman spectroscopy , materials science , surface enhanced raman spectroscopy , nanoparticle , biomolecule , cholesterol , nanotechnology , analytical chemistry (journal) , chemistry , optics , chromatography , physics , biochemistry
Cholesterol is an essential molecule for many processes in the human body and many diseases such as hypertension, coronary heart disease, atherosclerosis, and lipid metabolism dysfunction can be detected in their early stages by monitoring intracellular cholesterol levels. Herein, metallic surface‐enhanced Raman scattering (SERS) core–shell nanoparticles with an embedded standard are designed for nondestructive detection of H 2 O 2 and cholesterol. A classical least squares (CLS) spectral fitting method allows one to accurately extract the SERS signals of internal standards and target molecules from the multiplexed spectra. The embedded internal standard can be used to calibrate the SERS signals, removing fluctuations induced by differing measurement conditions and local states of the nanoparticles, such as aggregation. Thus, SERS signal reproducibility is improved and a better linear relationship in the working curve of detection is achieved. Using these SERS nanoprobes quantitative detection of H 2 O 2 and cholesterol in solution and inside live cells at a single‐cell level is demonstrated.