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A Trifunctional Linker for Purified 3D Assembled Peptide Structure Arrays
Author(s) -
Mattes Daniela S.,
Rentschler Simone,
Foertsch Tobias C.,
Münch Stephan W.,
Loeffler Felix F.,
NesterovMueller Alexander,
Bräse Stefan,
Breitling Frank
Publication year - 2018
Publication title -
small methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.66
H-Index - 46
ISSN - 2366-9608
DOI - 10.1002/smtd.201700205
Subject(s) - peptide , linker , combinatorial chemistry , azide , protein array analysis , matrix assisted laser desorption/ionization , biochip , mass spectrometry , chemistry , nanotechnology , materials science , dna microarray , chromatography , biochemistry , computer science , desorption , organic chemistry , gene expression , adsorption , gene , operating system
Microarrays are an important tool in modern research that allow the rapid screening of many different interactions simultaneously. Peptide arrays, which bear different peptides arranged in separate spots, permit high‐throughput screening to investigate linear and cyclic binding sites. To study conformational or discontinuous binding sites, protein arrays are the major choice. However, the tremendous costs for the generation of high‐density protein arrays of high purity restrict progress in protein research. Therefore, peptide‐based arrays, which can mimic assembled peptide structures, have an enormous potential. Here, a method is presented to create such structures in the array format as an alternative to protein arrays. A trifunctional linker is developed with an azide, a protected alkyne, and a carboxyl group, which can react with two or three different peptides. Due to the spatial proximity, the peptides interact and can form an assembled peptide structure. As a proof of concept, assembled peptide structures are demonstrated on beads and on a polymer surface and the approach can be validated via matrix‐assisted laser desorption/ionization spectrometry. Furthermore, a multistep transfer of peptide arrays is shown, generating purified assembled peptide structure arrays in high density.