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Challenges for Sensitive Quantification of Gene Editing “Off‐Target” Activity
Author(s) -
Tsai Shengdar Q.
Publication year - 2017
Publication title -
small methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.66
H-Index - 46
ISSN - 2366-9608
DOI - 10.1002/smtd.201600062
Subject(s) - genome editing , limit (mathematics) , sensitivity (control systems) , current (fluid) , gene , computational biology , computer science , biology , genetics , crispr , engineering , mathematical analysis , mathematics , electrical engineering , electronic engineering
Error rates of current high‐throughput sequencing technologies limit quantification of low‐frequency off‐target gene‐editing activity. The principles of gene editing are introduced and discussed, along with current challenges in targeted quantification and validation of low‐frequency gene‐editing off‐target sites and the detection of mutations with regard to improving current sensitivity limits.