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Integrated Addressable Dynamic Droplet Array (aDDA) as Sub‐Nanoliter Reactors for High‐Coverage Genome Sequencing of Single Yeast Cells (Small 37/2021)
Author(s) -
Li Chunyu,
Gong Yanhai,
Wang Xixian,
Xu Jian,
Ma Bo
Publication year - 2021
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.202170191
Subject(s) - nanotechnology , materials science , yeast , microfluidics , lysis , genome , whole genome sequencing , computational biology , biology , microbiology and biotechnology , genetics , gene
Single‐Cell Genome Sequencing In article number 2100325, Chunyu Li, Bo Ma, and co‐workers present an addressable dynamic droplet array (aDDA) that combines the advantages of static droplet arrays and continuous‐flow droplet platforms. All the sample preparation steps including single‐cell isolation, cell lysis, and amplification are performed in sequence within a sub‐nanoliter (≈300 pL) droplet, resulting in highly uniform whole genome amplification.