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Microfluidics: Direct and Label‐Free Cell Status Monitoring of Spheroids and Microcarriers Using Microfluidic Impedance Cytometry (Small 21/2021)
Author(s) -
Gong Lingyan,
Petchakup Chayakorn,
Shi Pujiang,
Tan Pei Leng,
Tan Lay Poh,
Tay Chor Yong,
Hou Han Wei
Publication year - 2021
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.202170101
Subject(s) - microcarrier , microfluidics , biomanufacturing , flow cytometry , spheroid , cell , nanotechnology , materials science , electrical impedance , microbiology and biotechnology , chemistry , cell culture , biology , immunology , engineering , electrical engineering , biochemistry , genetics
Non‐invasive and real‐time monitoring of microcarriers and three‐dimensional cell cultures are important in biomanufacturing and bioprocesses. In article number 2007500, Han Wei Hou and co‐workers introduce a low‐cost microfluidic impedance cytometer with Field's metal electrodes for label‐free and continuous‐flow monitoring of spheroids/microcarriers based on changes in impedance signatures. Potential applications include tracking of cell proliferation (biomass), cell viability and stem cell differentiation on hydrogel and commercial microcarriers.